Tissue-resident memory T (TRM) cells exist throughout the body where they are poised to mediate local immune responses. Although studies have defined a common mechanism of residency independent of location, there is likely to be a level of specialization that adapts TRM cells to their given tissue of lodgment. Here, we assessed the factors that contribute to the heterogeneity of TRM cell identity in diverse organs such as skin, liver, salivary glands and SI-IEL. We found that the phenotype and transcriptional profile of TRM cells is dictated by tissue-derived factors and that TRM cells in different locations differ in the requirements for their developmental and long-term survival. Importantly, we found that TRM cells relocated to different organs dynamically modified their phenotype and cytokine dependencies in line with their new location. As a consequence, these results argue for tissue-specific overlays to the TRM cell residency program, that is tailored to the particular tissue of TRM cell lodgment.